Department of Structural Biology Cryo-electron Microscopy (CryoEM) Facility

The Department of Structural Biology CryoEM Facility is furnished with state-of-the-art electron microscopy instrumentation and has full capability to carry out high resolution three-dimensional (3D) structural analysis of a diverse spectrum of specimens including cells and tissues, proteins, protein complexes, viruses, macromolecular assemblies, cellular organelles, and bacterial cells.  
The capabilities of the CryoEM Core include:

  1. CryoEM imaging of rapid-frozen biological samples in the native state (i.e., no fixation, no staining, no plastic embedding);
  2. High resolution 3D structural determination of large proteins, protein complexes, viruses, and macromolecular assemblies;
  3. 3D imaging and structural determination of unique objects such as native individual biological cells using cryo-electron tomography (cryo-ET);  
  4. Integrated imaging with correlative confocal live cell imaging and cryo-ET; and
  5. 3D structural determination of nano-materials, biopolymers and biomimetics, and biological tissues.

Cryo-EM Facility web site   Dr. Conway's website