The Department of Structural Biology CryoEM Facility is furnished with state-of-the-art electron microscopy instrumentation and has full capability to carry out high resolution three-dimensional (3D) structural analysis of a diverse spectrum of specimens including cells and tissues, proteins, protein complexes, viruses, macromolecular assemblies, cellular organelles, and bacterial cells.
The capabilities of the CryoEM Core include:
- CryoEM imaging of rapid-frozen biological samples in the native state (i.e., no fixation, no staining, no plastic embedding);
- High resolution 3D structural determination of large proteins, protein complexes, viruses, and macromolecular assemblies;
- 3D imaging and structural determination of unique objects such as native individual biological cells using cryo-electron tomography (cryo-ET);
- Integrated imaging with correlative confocal live cell imaging and cryo-ET; and
- 3D structural determination of nano-materials, biopolymers and biomimetics, and biological tissues.